Elimination of the Interference from Aminoglycoside Antibiotics in the Pyrogallol Red-Molybdate Protein Dye-Binding Assay
نویسندگان
چکیده
منابع مشابه
Aminoglycoside interference in the pyrogallol red-molybdate protein assay is increased by the addition of sodium dodecyl sulfate to the dye reagent.
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متن کاملExtent of aminoglycoside interference in the pyrogallol red-molybdate protein assay depends on the concentration of sodium oxalate in the dye reagent.
brane to ensure that the sample has wicked down the membrane properly. Lastly, the manufacturer recommends that the analysis be performed on urine concentrated to a total protein concentration of 15–20 g/L. In the case of the sample described above, urinalysis demonstrated the presence of 443 leukocytes, 162 erythrocytes, and 3 epithelial cells per high-power field. The presence of debris assoc...
متن کاملSerum protein binding of the aminoglycoside antibiotics.
The binding of four aminoglycoside antibiotics by human serum was investigated under controlled conditions of physiological pH and temperature, by means of an ultrafiltration technique. No serum binding was demonstrable for gentamicin, tobramycin, or kanamycin, whereas streptomycin was 35% bound. Previous conflicting studies are discussed, and some of the pharmacological implications are consid...
متن کاملInfluence of a variable differential function on the stone-growth-related urinary depletion effect.
sodium oxalate without SDS) by varying the concentration of the dye (0.03 or 0.12 mmol/L), sodium oxalate (0 or 2 mmol/L), and SDS (25 or 100 mg/L) in all combinations. For protein assays, we mixed 20 L of sample, calibrator, or water (blank) with 1 mL of PRM reagent and measured the absorbance at 600 nm after 30 min against the blank. The effects of the aminoglycosides on the absorbance readi...
متن کاملTotal protein determination in urine: elimination of a differential response between the coomassie blue and pyrogallol red protein dye-binding assays.
BACKGROUND The total protein content of urine is a good index of renal function, but its determination is unreliable. Protein dye-binding assays are simple, but they characteristically lack a uniform response to different proteins. METHODS We investigated a differential response of the Sigma Microprotein Coomassie Brilliant Blue (CBB) and Pyrogallol Red-molybdate (PRM) protein dye-binding ass...
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ژورنال
عنوان ژورنال: Clinical Chemistry
سال: 2004
ISSN: 0009-9147,1530-8561
DOI: 10.1373/clinchem.2004.036392